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pARO180 plasmid in <em>E. coli</em>
pARO180 plasmid in <em>E. coli</em>
規(guī)格:
貨期:
編號:B161526
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pARO180 plasmid in E. coli
商品貨號 B161526
Designations pARO180 plasmid in E. coli
Depositors D Parke
Biosafety Level 1
Host Escherichia coli DH5α
Vector Information
Size (kb): 5.46
Vector: pARO180 (plasmid)
Promoters: Promoter lac
Construction: pUC18, pSUP2021
Marker(s): ampR
Construct size (kb): 5.46
Cloning sites: HindIII SphI PstI HincII SalI XbaI BamHI AvaI SmaI XmaI KpnI SacI EcoRI
Distribution host: Escherichia coli DH5α
Suggested host: Escherichia coli S17-1 (ATCC® 47055™)
Possible hosts: Enterobacter aerogenes, Escherichia coli

Features:
insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1
Applications
Expression vector
Vector containing primer sites useful for sequencing
Vector permitting visual detection of recombinants
Vector useful for insertional mutagenesis
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--5.5; PstI--5.5; BamHI--5.5; EcoRI--5.5; HindIII/EcoRI--5.5.

Mobilizable expression vector encoding the lacZalpha (lacZ') peptide permitting visual detection of recombinants. Contains priming sites useful for sequencing.

Can be used as an expression vector in bacteria of the family Enterobacteriaceae, or as a mobilizable suicide vector for site-specific insertional mutagenesis in diverse other bacterial strains.

Replicates to high copy number in E. coli and Enterobacter aerogenes.

Contains the basis of mobilization (bom) and origin of transfer (oriT) sequences from RP4. This region can be transferred to another version of pUC vectors by using a MCS restriction site and the unique AlwNI site.

The order of the major features in this plasmid is: lacZ'/MCS - pMB1 ori - AlwNI site - bla - oriT/bom. pARO180 (ATCC® 77123™) and pARO190 (ATCC® 77124™) differ in the orientation of the MCS in lacZ'.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C
References

Parke D. Construction of mobilizable vectors derived from plasmids RP4, pUC18 and pUC19. Gene 93: 135-137, 1990. PubMed: 2227423

Shipped frozen
Shipping Information in E. coli containing the plasmid
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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