| 產(chǎn)品名稱 | CPAE |
|---|---|
| 商品貨號 | B164292 |
| Organism | Bos taurus, cow |
| Tissue | pulmonary artery/endothelium |
| Cell Type | endothelial |
| Product Format | frozen |
| Morphology | endothelial |
| Culture Properties | adherent |
| Biosafety Level | 2 [Cells contain Bovine Viral Diarrhea Virus (BVDV)]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | normal |
| Gender | female |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | normal female; diploid; stable |
| Images |  |
| Derivation | CPAE is an endothelial cell line derived from the main stem pulmonary artery of a young cow (Bos taurus). This line was initiated in January, 1979 by P. Del Vecchio from artery lumen scrapings dispersed by enzyme treatment. |
| Clinical Data | female |
| Genes Expressed | positive for angiotensin converting enzyme |
| Cellular Products | positive for angiotensin converting enzyme |
| Comments | Subsequent to the publication of the sixth edition of the ATCC catalog, tests for bovine diarrhea virus (BVD) have indicated that CPAE cells test positive for BVD viral antigen. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week
|
| Cryopreservation | culture medium 95%; DMSO, 5% |
| Culture Conditions | Temperature: 37°C
Atmosphere: 95% air; 5% CO2 |
| Name of Depositor | PJ Del Vecchio |
| Deposited As | Bos taurus |
| Year of Origin | 1979 |
| References | Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438 Girard JP, Springer TA. Modulation of endothelial cell adhesion by hevin, an acidic protein associated with high endothelial venules. J. Biol. Chem. 271: 4511-4517, 1996. PubMed: 8626806 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
