精品无码久久久久久久久,久久精品国产精品亚洲毛片,欧美黑人性暴力猛交喷水,久久精品国产久精国产,国产极品美女高潮无套在线观看,一区二区三区,国产精品视频一区二区三区不卡,亚洲国产精品一区二区成人片国内

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > GH4C1
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
GH4C1
GH4C1
規(guī)格:
貨期:
編號(hào):B164505
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 GH4C1
商品貨號(hào) B164505
Organism Rattus norvegicus, rat
Tissue pituitary
Product Format frozen
Morphology epithelial
Culture Properties loosely adherent with floating clusters
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease tumor
Age 7 months
Gender female
Strain Wistar-Furth
Applications
The GH4C1 cell line provides a system for studying the molecular mechanisms of receptor modulation and signal transduction in pituitary - derived cells.

These cells may be used for electrophysiological studies on plasma membrane calcium channels and also for studies of intracellular calcium homeostasis in secretory cells.

Cloned cDNA from GH4C1 cells has been used to investigate the structure and regulation of the rat TRH receptor.
Storage Conditions liquid nitrogen vapor phase
Karyotype modal number = 65; range = 60 to 68.
This is a hypertriploid rat cell line with the modal number of 65, occurring in 32% of cells. However, cells with 67 chromosomes also occurred at a high rate (22%). Cells with a higher ploidy count occurred at 0.6%. There were 25 to 30 marker chromosomes per cell including paired T(1;2-), single 4p+, chromosome 2 with the deleted q16-q24 (?), 11p+ and about 15 other constitutive markers. Only a single copy of the X chromosome was identified. Other characteristics included consistent single copies for chromosomes 1 and 3, double copies for chromosomes 5 and 8, and more than four copies for chromosome 7.
Derivation
The GH4C1 cell line was developed in 1972 by A.H. Tashjian, Jr. from serially propagated GH3 (ATCC CCL-82.1) cells that suddenly produced little or no detectable levels of growth hormone.
Clinical Data
female
7 months
Genes Expressed
prolactin; growth hormone (somatotrophin)
Cellular Products
prolactin; growth hormone (somatotrophin)
Comments

Note:  Cytogenetic information is based on initial seed stock at ATCC.  Cytogenetic instability has been reported in the literature for some cell lines.

GH4C1 cells differ from GH3 cells in that basal levels of growth hormone production are low (0.06 µg or less hormone/mg cell protein/24 hours).

Prolactin is also produced at a rate of 0.006 to 0.012 µg hormone/mg cell protein/24 hours.
Thyrotropin-releasing Hormone (TRH) stimulates prolactin synthesis and secretion.

These cells have a plating efficiency of approximately 22% in the recommended culture medium.
Complete Growth Medium Ham's F10 medium, 82.5%; horse serum, 15%; fetal bovine serum, 2.5%
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it.
  2. Add 2.0 to 3.0 mL of 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
  3. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  4. Add appropriate aliquots of the cell suspension to new culture vessels.
  5. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:20 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Name of Depositor AH Tashjian
Deposited As Rattus sp.
References

Tashjian AH Jr., et al. Establishment of clonal strains of rat pituitary tumor cells that secrete growth hormone. Endocrinology 82: 342-352, 1968. PubMed: 4951281

Dannies PS and Tashjian AH. Growth Hormone and Prolactin from Rat Pituitary Tumor cells. Tissue Culture: methods and applications. pp. 561-569, New York: Academic Press; 1973.

Takemoto H, et al. Adrenotropic activity of mammo-somatotropic tumors in rats and mice. I. Biologic aspects. Cancer Res. 22: 917-924, 1962. PubMed: 13984648

Tashjian AH Jr.. Clonal strains of hormone-producing pituitary cells. Methods Enzymol. 58: 527-535, 1979. PubMed: 218079

Zhao D, et al. Molecular cloning of a complementary deoxyribonucleic acid encoding the thyrotropin-releasing hormone receptor and regulation of its messenger ribonucleic acid in rat GH cells [published erratum appears in Endocrinology 132: 2658, 1993]. Endocrinology 130: 3529-3536, 1992. PubMed: 1317787

Albert PR, Tashjian AH Jr.. Relationship of thyrotropin-releasing hormone-induced spike and plateau phases in cytosolic free Ca2+ concentrations to hormone secretion. Selective blockade using ionomycin and nifedipine. J. Biol. Chem. 259: 15350-15363, 1984. PubMed: 6439720

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
蒙山县| 邯郸市| 吉安县| 邯郸市| 西宁市| 望江县| 故城县| 桦南县| 元朗区| 内江市| 饶平县| 井冈山市| 鸡东县| 武冈市| 竹山县| 商南县| 冀州市| 营口市| 建始县| 隆德县| 新闻| 中卫市| 广德县| 东港市| 阜康市| 珠海市| 莲花县| 毕节市| 高州市| 垣曲县| 怀宁县| 湾仔区| 大宁县| 铜梁县| 获嘉县| 蓬溪县| 惠东县| 绍兴县| 镇安县| 会宁县| 南溪县|