| 產(chǎn)品名稱 |
Hepa-1c1c7 |
| 商品貨號 |
B164597 |
| Organism |
Mus musculus, mouse |
| Tissue |
liver |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
hepatoma |
| Strain |
C57L |
| Applications |
This and mutant derivatives of it are useful for studying the aryl hydrocarbon (Ah) receptor and P450IA1 regulation.
This cell line is a suitable transfection host.
|
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
This is a derivative of the BW7756 mouse hepatoma that arose in a C57/L mouse. |
| Receptor Expression |
aryl hydrocarbon (Ah) |
| Genes Expressed |
cytochrome P450IA1 |
| Cellular Products |
cytochrome P450IA1 |
| Comments |
The cells are highly inducible for cytochrome P450IA1. |
| Complete Growth Medium |
Alpha minimum essential medium without nucleosides, 90%; fetal bovine serum, 10%
|
| Subculturing |
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
- Remove and discard culture medium.
-
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
-
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
-
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
-
Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Population Doubling Time |
18 hrs |
| Name of Depositor |
O Hankinson |
| Deposited As |
Mus musculus |
| References |
Bernhard HP, et al. Expression of liver phenotypes in cultured mouse hepatoma cells: synthesis and secretion of serum albumin. Dev. Biol. 35: 83-96, 1973. PubMed: 4362668
Hankinson O, et al. Genetic and molecular analysis of the Ah receptor and of Cyp1a1 gene expression. Biochimie 73: 61-66, 1991. PubMed: 1851644
Hankinson O. Single-step selection of clones of a mouse hepatoma line deficient in aryl hydrocarbon hydroxylase. Proc. Natl. Acad. Sci. USA 76: 373-376, 1979. PubMed: 106390
|
