| 產(chǎn)品名稱 |
K117 |
| 商品貨號 |
B164898 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Astrocytoma,sarcoma |
| Strain |
Strain:? (BALB/c X C57BL/6)F1 (B cell); BALB/c (myeloma) |
| Applications |
The antibody reacts with the human Thy-1 antigen, a glycoprotein (gp25) of relative molecular weight 25000 daltons. The antigen is expressed on variety of normal and neoplastic tissues and is present on most sarcomas and tumors of neuroectodermal origin. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against a human astrocytoma cell line; human Thy-1, gp25 glycoprotein |
| Cellular Products |
immunoglobulin; monoclonal antibody; against a human astrocytoma cell line; human Thy-1, gp25 glycoprotein |
| Comments |
Animals were immunized with the SK-MG-2 human astrocytoma cell line. The antibody reacts with the human Thy-1 antigen, a glycoprotein (gp25) of relative molecular weight 25000 daltons. The antigen is expressed on variety of normal and neoplastic tissues and is present on most sarcomas and tumors of neuroectodermal origin. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 92.5%; DMSO, 7.5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG1 |
| Name of Depositor |
Sloan-Kettering Institute for Cancer Research |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Rettig WJ, et al. Cell-surface glycoproteins of human sarcomas. US Patent 5,059,523 dated Oct 22 1991
Rettig WJ, et al. Differential expression of cell surface antigens and glial fibrillary acidic protein in human astrocytoma subsets. Cancer Res. 46: 6406-6412, 1986. PubMed: 2877731
Rettig WJ, et al. Role of human chromosome 11 in determining surface antigenic phenotype of normal and malignant cells. Somatic cell genetic analysis of eight antigens, including putative human Thy-1. J. Exp. Med. 162: 1603-1619, 1985. PubMed: 2865325
Rettig WJ, et al. Differential expression of the human Thy-1 gene in rodent-human somatic cell hybrids [corrected] [published erratum appears in J. Immunol. 139: 1747, 1987]. J. Immunol. 138: 4484-4489, 1987. PubMed: 2884261
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