| 產(chǎn)品名稱(chēng) |
KM114 |
| 商品貨號(hào) |
B164919 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Animals were immunized with the BMS2 bone marrow derived stromal cell line. KM114 recognizes an epitope close to, but distinct from, those detected by KM201 and KM81 (see ATCC TIB-240 and ATCC TIB-241). |
| Derivation |
Animals were immunized with the BMS2 bone marrow derived stromal cell line. Spleen cells were fused with Sp2/0 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against the functional domain of mouse Pgp-1 (CD44) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against the functional domain of mouse Pgp-1 (CD44) |
| Comments |
Animals were immunized with the BMS2 bone marrow derived stromal cell line. Spleen cells were fused with Sp2/0 myeloma cells. The antibody blocks adhesion of a B cell hybridoma (BM-2) to BMS2 cells or to hyaluronate coated dishes. The antibody prevents growth of lymphoid and myeloid cells in long term bone marrow cultures. KM114 recognizes an epitope close to, but distinct from, those detected by KM201 and KM81 (see ATCC TIB-240 and ATCC TIB-241). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
- fetal bovine serum to a final concentration of 10%
- 0.05 mM 2-mercaptoethanol
|
| Subculturing |
Medium Renewal: Every 2 to 4 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
PW Kincade |
| Deposited As |
rat (B cell); mouse (myeloma) |
| References |
Miyake K, et al. Monoclonal antibodies to Pgp-1/CD44 block lympho-hemopoiesis in long-term bone marrow cultures. J. Exp. Med. 171: 477-488, 1990. PubMed: 2406365
Miyake K, et al. Hyaluronate can function as a cell adhesion molecule and CD44 participates in hyaluronate recognition. J. Exp. Med. 172: 69-75, 1990. PubMed: 2193100
|
