| 產(chǎn)品名稱 |
MOLT-3 |
| 商品貨號 |
B165183 |
| Organism |
Homo sapiens, human |
| Tissue |
peripheral blood |
| Cell Type |
T lymphoblast |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
acute lymphoblastic leukemia |
| Age |
19 years |
| Gender |
male |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
This is a hypertetraploid human cell line. The modal chromosome number was 97, occurring in 48% of cells. The rate of cells with higher ploidies was 2%. Three markers were common to all cells. They are: ?del(6) (q21), t(7;7), and an unidentifiable M5. The der(6) generally had three copies per cell, and the i(7q), two copies. The majority of normal chromosomes had 4 copies per cell. N8, N17, N19 and N20 had more than 4 copies in many cells. The X and Y chromosomes were two copies each per cell. Neither HSR chromosomes nor DM's were detected. |
| Derivation |
MOLT-3 was derived from the same patient as the MOLT-4 cell line (ATCC CRL-1582). This line was established from cells taken from a patient in relapse. |
| Clinical Data |
male
19 years |
| Antigen Expression |
CD1 (65%), CD4 (66%), CD5 (97%), CD7 (97%) |
| Genes Expressed |
terminal deoxynucleotidyl transferase (TdT) activity is high |
| Cellular Products |
terminal deoxynucleotidyl transferase (TdT) activity is high |
| Comments |
No immunoglobulin or Epstein-Barr virus is detectable.
The patient had received prior multidrug chemotherapy.
|
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Cultures are maintained by addition or replacement of fresh medium. Establish new cultures at 5 X 105 viable cells/mL and subculture at between 1 and 2 X 106 cells/mL.
Medium Renewal: Every 3 to 4 days |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C |
| Isoenzymes |
AK-1, 0 ES-D, 1 G6PD, B GLO-I, 1 Me-2, 0 PGM1, 1 PGM3, 0 |
| Name of Depositor |
J Minowada |
| Deposited As |
Homo sapiens |
| References |
Minowada J, et al. Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. J. Natl. Cancer Inst. 49: 891-895, 1972. PubMed: 4567231
Mertelsmann R, et al. T-cell growth factor (interleukin 2) and terminal transferase activity in human leukemias and lymphoblastic cell lines. Blut 43: 99-103, 1981. PubMed: 6942897
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