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The cells are killed by 10 nM dexamethasone.

Initial recovery from the frozen state is slow, and approximately 7 to 10 days are required before any noticeable cell growth occurs.

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10⁵ cells/mL and maintain between 1 x 10⁵ and 1 x 10⁶ viable cells/mL.

Complete culture medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Gehring U, et al. Glucocorticoid action on hybrid clones derived from cultured myeloma and lymphoma cell lines. Proc. Natl. Acad. Sci. USA 69: 3124-3127, 1972. PubMed: 4343956

Halpern MS, Coffman RL. Polymer formation and J chain synthesis in mouse plasmacytomas. J. Immunol. 109: 674-680, 1972. PubMed: 5074326

Potter M. Immunoglobulin-producing tumors and myeloma proteins of mice. Physiol. Rev. 52: 631-719, 1972. PubMed: 4555516

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.