| 產(chǎn)品名稱 |
NCI-H1155 [H1155] |
| 商品貨號(hào) |
B165252 |
| Organism |
Homo sapiens, human |
| Tissue |
lung; derived from metastatic site: lymph node |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
clusters in suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
carcinoma; non-small cell lung cancer |
| Age |
36 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Derivation |
This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a lymph node metastasis obtained from a patient prior to therapy. |
| Clinical Data |
36 years
Caucasian
male
|
| Genes Expressed |
neuromedin B (NMB) |
| Cellular Products |
neuromedin B (NMB) |
| Comments |
The cells are able to synthesize the peptide NMB at 0.2 pmol/mg protein, but not the gastrin releasing peptide (GRP). |
| Complete Growth Medium |
The base medium for this cell line is RPMI-1640 Medium (ATCC 30-2001).To make the complete growth medium, add the following components to the base medium: Fetal Bovine Serum (FBS; ATCC 30-2020) to a final concentration of 5%. |
| Subculturing |
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation of the suspension with subsequent resuspension in fresh medium. Add medium as the cell density increases.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 12 D13S317: 14 D16S539: 9 D5S818: 10 D7S820: 8 THO1: 6 TPOX: 8 vWA: 15 |
| Name of Depositor |
AF Gazdar, JD Minna |
| Deposited As |
Homo sapiens |
| References |
Giaccone G, et al. Neuromedin B is present in lung cancer cell lines. Cancer Res. 52: 2732s-2736s, 1992. PubMed: 1563005
NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
|
