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Incubation: 12-14 days at 30°C, a 5% CO₂ in air atmosphere is recommended.

Recommendations for Infection: For best results cells should be 24 to 48 hours old and 80% - 90% confluent (not 100% confluent).

Plotkin SA, et al. Studies of immunization with living rubella virus. Trials in children with a strain cultured from an aborted fetus. Am. J. Dis. Child. 110: 381-389, 1965. PubMed: 5834207

Plotkin SA, et al. Attenuation of RA 27/3 rubella virus in WI-38 human diploid cells. Am. J. Dis. Child. 118: 178-185, 1969. PubMed: 5794813