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Construct size (kb): 37.82300186157227

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: svPHEP
Intact vector size: 9.201
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI ClaI
Polylinker sites: HindIII ClaI EcoRI BamHI
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
replicon: pMB1
Cross references: DNA Seq. Acc.: L19900

DNA: genomic

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ABL1
Other gene symbols: ABL
Genomic copy number: unique
Gene name: Abelson murine leukemia viral (v-abl) oncogene homolog 1
Contains complete coding sequence?: N
Chromosome: 9; Localization: 9 q34.1
Tissue: lung carcinoma
Type of DNA: genomic
Insert end: MboI
Insert end: MboI
Insert size (kb): 28.622
Cross references: DNA Seq. Acc.: U07563
Nucleotides 1-29622 of the insert correspond to
nucleotides 44062-73683 of U07563.

Insert lengths(kb): 28.62199974060059

Tissue: lung carcinoma

Gene product: Abelson murine leukemia viral (v-abl) oncogene homolog 1 [ABL]

Alleles: D1, D2, D1, D2, A1, A2, B2, C1, C2, A1, A2, B1, B2, C1, C2, B1

Restriction digests of the clone give the following sizes (kb): BamHI--15.5, 12.5, 7.7, 3.6, 0.65; BglII--8.0, 7.7, 7.0, 4.6, 4.4, 3.1, 2.55, 2.3, 1.75, 1.5, 0.68; EcoRI--7.7 (doublet), 6.2, 3.4 (doublet), 3.1, 2.65, 2.3, 1.85, 1.15, 0.84;

HindIII--9.2, 7.7, 5.7, 4.6, 3.3, 3.0, 2.65, 1.8, 1.3, 0.98; NotI-->23.0; SstI--8.0, 7.7, 4.1 (doublet), 3.4, 3.1, 2.4, 2.3, 2.0, 1.55, 1.15.

IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.

The insert begins in intron 1a and extends into intron 8.