| 產(chǎn)品名稱 |
pgsB-618 |
| 商品貨號(hào) |
B165503 |
| Organism |
Cricetulus griseus, hamster, Chinese |
| Tissue |
ovary |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
galactosyltransferase I deficient |
| Gender |
female |
| Applications |
PgsB-618 is a Chinese hamster ovary cell mutant deficient in galactosyltransferase I (UDP-D-galactose:xylose-1,4-D-galactosyl- transferase). The cell line was derived from CHO-K1 cells (see ATCC CCL-61) treated with mutagen (ethylmethanesulfonate) and screened for mutants defective in proteoglycan synthesis. PgsB-618 cells do not produce glycosaminoglycans. |
| Derivation |
The cell line was derived from CHO-K1 cells (see ATCC CCL-61) treated with mutagen (ethylmethanesulfonate) and screened for mutants defective in proteoglycan synthesis. |
| Clinical Data |
female |
| Comments |
PgsB-618 is a Chinese hamster ovary cell mutant deficient in galactosyltransferase I (UDP-D-galactose:xylose-1,4-D-galactosyl- transferase). The cell line was derived from CHO-K1 cells (see ATCC CCL-61) treated with mutagen (ethylmethanesulfonate) and screened for mutants defective in proteoglycan synthesis. PgsB-618 cells do not produce glycosaminoglycans. |
| Complete Growth Medium |
Ham's F12 medium, 90%; fetal bovine serum, 10%
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: Every 2 to 3 days Rinse the cell sheet with fresh 0.25% trypsin, 0.03% EDTA solution, add an additional 1 to 2 ml of trypsin solution and allow the culture to sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Name of Depositor |
JD Esko |
| Deposited As |
Cricetulus griseus |
| References |
Esko JD, et al. Tumor formation dependent on proteoglycan biosynthesis. Science 241: 1092-1096, 1988. PubMed: 3137658
Esko JD, et al. Inhibition of chondroitin and heparan sulfate biosynthesis in Chinese hamster ovary cell mutants defective in galactosyltransferase I. J. Biol. Chem. 262: 12189-12195, 1987. PubMed: 2957376
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