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XC1.5/51
XC1.5/51
規(guī)格:
貨期:
編號:B165968
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 XC1.5/51
商品貨號 B165968
Organism Mus musculus, mouse
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease plasmacytoma; myeloma
Strain C3H
Applications
The cells do not synthesize or secrete immunoglobulin.
Tested and found negative for ectromelia virus (mousepox).
Karyotype near tetraploid
Derivation
A derivative of C1.18 (ATCC TIB-11).
Comments
A derivative of C1.18 (ATCC TIB-11).
The cells do not synthesize or secrete immunoglobulin.
The cells do not express PC.1 or H-2 k.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Name of Depositor K Horibata, D Schubert
Deposited As Mus musculus
References

Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579

Hyman R, et al. Cell-specific antigens and immunoglobulin synthesis of murine myeloma cells and their variants. J. Natl. Cancer Inst. 48: 173-184, 1972. PubMed: 4119881

Herberman RB. Serological analysis of cell surface antigens of tumors induced by murine leukemia virus. J. Natl. Cancer Inst. 48: 265-271, 1972. PubMed: 4119883

Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714

Watson J, et al. Leukemia viruses associated with mouse myeloma cells. Proc. Natl. Acad. Sci. USA 66: 344-351, 1970. PubMed: 4317914

Schubert D, Horibata K. Immunoglobulin biosynthesis. II. Four independently isolated myeloma variants. J. Mol. Biol. 38: 263-271, 1968. PubMed: 4180612

Schubert D, Cohn M. Immunoglobulin biosynthesis. 3. Blocks in defective synthesis. J. Mol. Biol. 38: 273-288, 1968. PubMed: 4180613

J. Natl. Cancer Inst. 19: 371, 1957.

Kimmel CB. On the RNA in cultured myeloma cells producing immunoglobulin. Biochim. Biophys. Acta 182: 361-374, 1969. PubMed: 4183552

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