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Galpha Q209L HA [alpha q-Q209L (mouse)]
Galpha Q209L HA [alpha q-Q209L (mouse)]
規(guī)格:
貨期:
編號(hào):B226566
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Galpha Q209L HA [alpha q-Q209L (mouse)]
商品貨號(hào) B226566
Designations Galpha Q209L HA [alpha q-Q209L (mouse)]
GenBank Number

M55412

Species Mus musculus, mouse
Depositors HR Bourne, P Herzmark
Applications
in another host, produces protein guanine nucleotide binding protein (G protein), q polypeptide heterotrimeric guanine nucleotide-binding protein alpha q subunit, G alpha q
Vector
Construct size (kb): 5.199999809265137
Insert
DNA: cDNA
Insert lengths(kb): 1.200000047683716
Gene product: guanine nucleotide binding protein (G protein), q polypeptide( heterotrimeric guanine nucleotide-binding protein alpha q subunit, G alpha q) [Gnaq]
Target Gene: guanine nucleotide binding protein (G protein), q polypeptide
Insert Size (kb) 1.200
Media ATCC® Medium 1890: LB medium (ATCC medium 1065) with 12 mcg/ml tetracycline and 100 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.2; EcoRI--5.2; HindIII--5.2; PstI--5.2; XbaI--5.2; XhoI--5.2.
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.75; BglII--0.93; EcoRI--1.02; NdeI--0.48; NotI--0.04; PstI--0.08, 1.15; PvuII--1.15; SacI--0.22; SalI--0.26, 0.97; SmaI--0.02; XbaI--0.94; XhoI--1.02.
Deposited with the understanding that the DNAs not be used in human experiments.
The protein produced is the Q209L mutation (leucine replacing glutamine at codon 209) with a hemagglutinin tag.
Nucleotides 93-95 and 687 of the GenBank sequence have been altered to add restriction sites for SalI and BamHI respectively. This does not alter the amino acid sequence.
Encodes a hemagglutinin epitope replacing nt 409-426 of the GenBank sequence.
Restriction site(s) at the 3' end of the insert for linearizing the construct: NsiI.
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Wedegaertner PB, et al. Palmitoylation is required for signaling functions and membrane attachment of Gq-alpha and Gs-alpha. J. Biol. Chem. 268: 25001-25008, 1993. PubMed: 8227063

Paul Herzmark, personal communication

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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