精品无码久久久久久久久,久久精品国产精品亚洲毛片,欧美黑人性暴力猛交喷水,久久精品国产久精国产,国产极品美女高潮无套在线观看,一区二区三区,国产精品视频一区二区三区不卡,亚洲国产精品一区二区成人片国内

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > Euglena gracilis Klebs
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
Euglena gracilis Klebs
Euglena gracilis Klebs
規(guī)格:
貨期:
編號:B227259
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Euglena gracilis Klebs
商品貨號 B227259
Strain Designations Z etiolated
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
material from Saedeleer, 1950
Product Format frozen
Type Strain no
Medium ATCC® Medium 351: Hutner's medium for Euglena
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Protocol: plus 0.1% sodium acetate
Subcultivation
Protocol: plus 0.1% sodium acetate
Cryopreservation

1.?? Harvest cells from a culture which is at or near peak density by centrifuging at 100 x g for 1 minute. Note: Centrifugation at the lowest speed and the shortest time to allow sedimentation of the cells will maximize recovery.?

2.?? Adjust the concentration of cells to 4 x 106/ml with fresh broth medium.

3.?? Transfer the concentrated cell suspension to a sterile Petri dish and allow the cells to remain undisturbed for at least one hour.?

4.?? Transfer the cell suspension (note the volume) from the Petri plate to a 15 ml plastic centrifuge tube.?

5.?? Add an equal volume of 6% (v/v) sterile reagent grade methanol solution that has been prepared in fresh ATCC medium 351.

6. ? Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).? The time from mixing of the cell preparation and the methanol solution, before the cooling cycle begins, should be no greater than 15 min.

7.?? Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.? Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.? (The cooling rate in this apparatus is approximately -1°C/min.) ??

8.?? The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials can be stored between -80 and -70°C for no longer than one week.

9.?? To establish a culture from the frozen state follow steps 1-4 listed above under the heading ESTABLISHING A CULTURE FROM A FROZEN AMPULE.

Name of Depositor RG Yaeger
Chain of Custody
ATCC <<--RG Yaeger<<--UTEX 753 <<--- E.G. Pringsheim
Year of Origin 1950
References

Helmy FM, et al. Comparative lipid biochemistry. VI. Lipids of green and etiolated Euglena gracilis and of Blastocrithidia culicis. Comp. Biochem. Physiol. 23: 565-567, 1967. PubMed: 6080513

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
昭苏县| 荔浦县| 沾益县| 仁化县| 淮南市| 辽阳市| 华池县| 江口县| 磐安县| 靖远县| 长海县| 集贤县| 渑池县| 遂溪县| 兖州市| 洛隆县| 江门市| 措勤县| 克什克腾旗| 治多县| 喜德县| 嘉鱼县| 阿拉尔市| 福贡县| 东兴市| 健康| 抚顺县| 闽清县| 宁夏| 华池县| 会东县| 改则县| 兴城市| 南平市| 甘洛县| 太原市| 宁国市| 剑川县| 普兰店市| 普兰县| 耿马|